Comparison of autoclaving and γ-radiation impact on four spices aroma profiles and microbial load using HS-SPME GC-MS and chemometric tools.

Herbal spices are widely consumed as food additives owing to their distinct aroma and taste as well as a myriad of economic and health value. The aroma profile of four major spices including bay leaf, black pepper, capsicum, and fennel was tested using HS-SPME/GC-MS and in response to the most widely used spices´ processing methods including autoclaving and γ-radiation at low and high doses. Additionally, the impact of processing on microbial contamination of spices was tested using total aerobic count. GC-MS analysis led to the identification of 22 volatiles in bay leaf, 34 in black pepper, 23 in capsicum, and 24 in fennel. All the identified volatiles belonged to oxides/phenols/ethers, esters, ketones, alcohols, sesquiterpene and monoterpene hydrocarbons. Oxides/phenol/ethers were detected at high levels in all tested spices at ca. 44, 28.2, 48.8, 61.1%, in bay leaves, black pepper, capsicum, and fennel, respectively of the total blend and signifying their typical use as spices. Total oxides/phenol/ethers showed an increase in bay leaf upon exposure to γ-radiation from 44 to 47.5%, while monoterpene hydrocarbons were enriched in black pepper upon autoclaving from 11.4 in control to reach 65.9 and 82.6% for high dose and low dose of autoclaving, respectively. Cineole was detected in bay leaf at 17.9% and upon exposure to autoclaving at high dose and γ-radiation (both doses) its level increased by 29-31%. Both autoclaving and γ-radiation distinctly affected aroma profiles in examined spices. Further, volatile variations in response to processing were assessed using multivariate data analysis (MVA) revealing distinct separation between autoclaved and γ-radiated samples compared to control. Both autoclaving at 115 °C for 15 min and radiation at 10 kGy eliminated detected bioburden in all tested spices i.e., reduced the microbial counts below the detection limit (< 10 cfu/g).

New monoterpene phenol dimers from the fruits of Psoralea corylifolia.

Three new monoterpene phenol dimers, bisbakuchiols V-X (1-3), and two bakuchiol ethers (4 and 5), along with four known compounds (6-9) were isolated from the fruits of Psoralea corylifolia. Their structures were elucidated based on extensive spectral analysis. The absolute configurations of 1, 2, 4, and 5 were specified by quantum chemical calculations of ECD spectra.

Insights into kinetics, thermodynamics, and mechanisms of chemically activated sunflower stem biochar for removal of phenol and bisphenol-A from wastewater.

This study synthesized a highly efficient KOH-treated sunflower stem activated carbon (KOH-SSAC) using a two-step pyrolysis process and chemical activation using KOH. The resulting material exhibited exceptional properties, such as a high specific surface area (452 m2/g) and excellent adsorption capacities for phenol (333.03 mg/g) and bisphenol A (BPA) (365.81 mg/g). The adsorption process was spontaneous and exothermic, benefiting from the synergistic effects of hydrogen bonding, electrostatic attraction, and stacking interactions. Comparative analysis also showed that KOH-SSAC performed approximately twice as well as sunflower stem biochar (SSB), indicating its potential for water treatment and pollutant removal applications. The study suggests the exploration of optimization strategies to further enhance the efficiency of KOH-SSAC in large-scale scenarios. These findings contribute to the development of improved materials for efficient water treatment and pollution control.

Site-scale groundwater pollution risk assessment using surrogate models and statistical analysis.

Petroleum pollution in soil and groundwater has emerged as a significant environmental concern worldwide. As a sustainable and cost-effective in-situ remediation technique, Monitored Natural Attenuation (MNA) exhibits significant promise in addressing sites contaminated by petrochemicals. This study specifically targets a typical petrochemical-contaminated site in northern China and employs GMS software to establish a comprehensive physical model. The model relies on time-series monitoring data of phenol concentrations spanning from 2018 to 2020, effectively simulating both the leakage and natural attenuation of phenol. Within this study, the adsorption coefficient and maximum adsorption capacity emerge as the foremost influential factors shaping the outcomes of the model. Given the inherent heterogeneity of the site and the variability of hydrochemical conditions, parameters such as dispersion, porosity, and adsorption coefficient exhibit significant uncertainties. Consequently, relying on traditional deterministic models to predict the feasibility of MNA technology is not reliable. Therefore, this study employs machine learning (ML) methods to construct stochastic parameter models based on physical processes. The Random Forest Regression (RFR) algorithm, after trained, demonstrates strong alignment with numerical model output, exhibiting an average Nash-Sutcliffe Efficiency (NSE) >0.96. Using a stochastic approach, RFR iteratively computes phenol concentration across 6000 sets of parameters. Applying probability statistics, the model shows a notable reduction in the likelihood of phenol concentrations exceeding a threshold, dropping from 64.0% to 15.7% before and after natural attenuation. In parameter uncertainty, the stochastic model emphasizes natural attenuation's efficacy in mitigating phenol pollution risk (porosity being the most influential factor). This case study proposed a novel method to quickly assess the pollution risks at petrochemical sites under the influence of the uncertainty of pollutant transport and reaction parameters. The results can provide a reference for the pollution risk assessment at petrochemical sites, especially in sites with high stratigraphic heterogeneity or insufficient transport parameter data.

Efficient flocculation pretreatment of coal gasification wastewater by halophilic bacterium Halovibrio variabilis TG-5.

The isolated halophilic bacterial strain Halovibrio variabilis TG-5 showed a good performance in the pretreatment of coal gasification wastewater. With the optimum culture conditions of pH = 7, a temperature of 46 °C, and a salinity of 15%, the chemical oxygen demand and volatile phenol content of pretreated wastewater were decreased to 1721 mg/L and 94 mg/L, respectively. The removal rates of chemical oxygen demand and volatile phenol were over 90% and 70%, respectively. At the optimum salinity conditions of 15%, the total yield of intracellular compatible solutes and the extracellular transient released yield under hypotonic conditions were increased to 6.88 g/L and 3.45 g/L, respectively. The essential compatible solutes such as L-lysine, L-valine, and betaine were important in flocculation mechanism in wastewater pretreatment. This study provided a new method for pretreating coal gasification wastewater by halophilic microorganisms, and revealed the crucial roles of compatible solutes in the flocculation process.

Dual optical detection approach for capillary electrophoresis following two-step liquid-liquid extraction to determine ten phenols in water samples.

In this research, the analytical method was developed and evaluated for determining phenol and its nine derivatives belong to the US EPA priority pollutant list in water samples by using dual-channeled capillary electrophoresis (CE) coupled with two types of optical detectors, namely LED-induced fluorescence (LEDIF) and ultraviolet (UV) detectors. The optimal background electrolytes for the first and second CE channels were 20 mM borate (pH 9.80) with 400 µM fluorescein and 55 mM borate (pH 11.75), respectively. The two-step liquid-liquid extraction (LLE) was used for sample preparation and enrichment, in which phenol and its derivatives were extracted from the aqueous phase using 10 mL of n-hexane/1-octanol (60/40, v/v) and then were back extracted into a 0.1 M NaOH as a final acceptor phase. Under the optimal CE and two-step LLE conditions, the enrichment factors of 10 phenols were 184 - 1120-fold, and the method detection limits were lowered to 0.02-0.60 µg/L. The obtained intra-day and inter-day precisions in terms of relative standard deviations (RSD) were between 4.0 and 7.3 % and 6.7 and 14 %, respectively. This approach was used to determine phenols in water samples, with recoveries ranging from 82.0 to 108.9 %. In combination with sample enrichment by two-step LLE extraction, this is the first CE study conducted to determine phenols in the EPA list using two detector approaches, specifically CE-LEDIF/CE-UV.

The Chemistry Behind the Folin-Ciocalteu Method for the Estimation of (Poly)phenol Content in Food: Total Phenolic Intake in a Mediterranean Dietary Pattern.

The Folin-Ciocalteu assay is a reference method for the quantification of total (poly)phenols in food. This review explains the fundamental mechanism of the redox reaction on which the method is based and looks at some of the practical considerations concerning its application. To accurately estimate the antioxidant capacity of (poly)phenolic compounds, a thorough knowledge of their structural characteristics is essential, as the two are closely associated. Therefore, to help researchers interpret the results of the Folin-Ciocalteu method, this review also summarizes some of the main phenolic structural features. Finally, we have used the Folin-Ciocalteu method to estimate the total phenolic intake associated with high adherence to a Mediterranean diet, ranked as one of the healthiest dietary patterns, which is characterized by a high consumption of (poly)phenol-rich food such as wine, virgin olive oil, fruits, vegetables, whole grains, nuts, and legumes.

Photocatalytic evaluation of synthesized MnO2/Fe3O4 NCs by Q. infectoria extract for removal Ni(II) and phenol: Study phytochemical, kinetics, thermodynamics, and antibioactivity.

In the present study, the plant extract of the Quercus infectoria galls was used as a reducing, capping, and stabilizer agent for green synthesized MnO2 nanoparticles (NPs) and MnO2/Fe3O4 nanocomposites (NCs) due to its reduction ability from polyphenol and antioxidant content. The green synthesized nanomaterials have been characterized by various techniques such as FTIR, UV-vis, XRD, SEM, EDS, and TEM. The average size of about 7.4 and 6.88 nm was estimated for the NCs crystals of SEM images and XRD analysis by the Scherrer and Williamson-Hall methods. The green synthesized MnO2/Fe3O4 NCs (dosage: 0.1 g) have shown high photocatalytic activity for the removal of Ni(II) in acidic and basic solutions under visible irradiation (220 V lamp). The removal efficiency for the Ni(II) solution (3.6 × 10-3 M) at pH = 3 was increased to pH = 12 from 56 % to 98 %. The oxidase-like activity of MnO2/Fe3O4 NCs at different dosages (0.05, 0.1, and 0.15 g) for the removal and colorimetric of phenol (1 g/40 mL) in the presence 4-AAp (1 g) was seen after only 28, 13, and 5 s, respectively. The kinetic evaluation results showed the pseudo-second-order kinetics model closely matched the adsorption capacity theoretical values qe,cal (578.03, 854.70, 892.85, and 917.43 mg.g-1) and experimental values qe,exp (521.84, 839.74, 887.86, and 913.22 mg.g-1) at different initial pH solution (3-12) for Ni(II) removal. In addition, the investigation of isotherm models revealed that the Langmuir model (R2 = 0.9955) explains a better estimate for a monolayer and favorable removal of Ni(II) ions onto NCs. Also, the low Temkin constant, BT < 0 (0.0200 kJ.mol-1), and positive ∆H° value (0.103 kJ.mol-1.K-1) illustrated that Ni(II) removal is physical sorption and endothermic process. However, the obtained thermodynamic results showed the negative values ΔG° with the increase in temperature (303-318 K) toward a spontaneous removal process of Ni(II). Finally, the plant antioxidant (200 to 3200 µg/mL) and antimicrobial activities (0.001 to 0.1 g/mL) for plant extract, MnO2 NPs, and MnO2/Fe3O4 NCs were evaluated against Gram-positive and Gram-negative bacteria species.

Analysis and derivation of the marine water quality criteria of phenol for Korean seas.

Marine water quality criteria (WQC) have to be determined prior to the derivation of water quality based effluent limitations (WQBELs) for hazardous and noxious substances (HNS) discharged from marine industrial facilities. In this study, we carried out toxicity tests using ten native marine organisms and analyzed international toxicity data and data tested in this study to derive the WQC of phenol for Korean seas. By converting acute values to chronic ones with ACRs (acute-chronic ratios) of each trophic level according to well-verified method, we derived provisional WQC (0.96 mg/L) of phenol for Korean seas for the first time. The procedure to derive marine WQC and results of this study could provide the essential information for the establishment of national marine WQC and WQBELs for HNS discharged from marine industrial facilities.

Multifunctional polysaccharide structure as green adsorbent for efficient removal and preconcentration of chlorophenols from the aqueous medium: experimental and modeling approaches.

The growing concerns about water pollution have prompted researchers to explore new materials for remediating and purifying it. In recent years, there has been a focus on polysaccharides as eco-friendly polymers that exhibit high efficiency in removing chlorophenols from waste water. This study aims to develop a trifunctional polysaccharide structure using a biodegradable matrix. The chitosan/alginate-polyethyleneimine-phenyl-phosphonamidic acid (CHIT/ALG-PEIPPAA) matrix was employed for removing chlorophenols from water. The study carefully examined the impact of various physicochemical parameters such as pH, reaction time, chlorophenols concentration, temperature, and ionic strength to determine the optimal conditions for the adsorption process. Several techniques were used to confirm the morphology, physicochemical properties, structure, and functionalization of the polymer. Scanning electron microscopy (SEM) images revealed a heterogeneous morphology with agglomerates of different particle sizes, ranging from a few micrometers with irregular shapes. The FTIR spectrum and zeta potential characterization indicated the presence of hydrophilic groups and a highly positive charge (around 31.4 mV) on the surface of the CHIT/ALG-PEIPPAA adsorbent. The optimal pH for chlorophenols removal was found to be approximately 4.4. The kinetic data supported the pseudo-second-order kinetic model, which accurately described the adsorption behavior of both chlorophenol molecules. The fitting of the isotherm analysis revealed that the Langmuir model provided a better representation of the adsorption process. The maximum adsorption capacities for 4-chlorophenol and 2,4-chlorophenol were approximately 118 mg.g-1 and 249 mg.g-1, respectively. The calculated thermodynamic functions confirmed an exothermic and spontaneous adsorption process for chlorophenols, with ∆H values of -6.98 kJ.mol-1 and -2.74 kJ.mol-1 for 4-chlorophenol and 2,4-chlorophenol, respectively. The regeneration process of the CHIT/ALG-PEIPPAA adsorbent showed higher efficacy in the presence of hydrochloric acid (2.0 mol.L-1), resulting in up to 91% desorption of chlorophenols. The CHIT/ALG-PEIPPAA adsorbent demonstrated good reusability after regeneration, with only a slight decrease in extraction efficiency: 34.63% for 4-chlorophenol and 79.03% for 2,4-chlorophenol, under the same optimal conditions as the initial adsorption cycle.

White rice enrichment with phenols upon cooking in olive leaf infusion: a preliminary study.

BACKGROUND: White rice is poor in health-promoting phytochemicals; therefore, the production of a phenol-enriched commodity is highly desirable. Recent findings on its enrichment via cooking in plant extracts are promising, yet studies employing aqueous extracts of olive leaves (OLs), containing well-recognized bioactive phenols (e.g. oleuropein) are absent. In addition, little is known about the levels of phenols that are maintained after rice drying and rehydration, an important aspect for the future design of 'ready-to-eat' functional rice. RESULTS: The examination, for the first time, of white rice adsorption capacity of phenols from OLs upon cooking in infusions containing different levels of phenols, after freeze-drying and rehydration, showed the following: (i) the total phenol content, the antioxidant activity (assessed via 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing antioxidant power assays), the oleuropein and luteolin-7-O-glucoside levels increased dose dependently; (ii) upon rehydration, the average decrease of total phenol content and antioxidant activity values was significantly lower when an exact volume of water was used compared with an excess (~10% versus 63%). A similar trend was observed for oleuropein (36% versus 83%) and the luteolin-7-O-glucoside (24 versus 82%) levels; (iii) the dried enriched kernels were less bright with a hay-yellow hue (CIELab coordinates). CONCLUSION: White rice enrichment with biophenols from OLs, a by-product of olive tree cultivation, was successful using a simple approach. Despite leaching upon freeze-drying/rehydration, sufficient amounts were maintained to obtain a functional rice that could serve as an alternative dietary source of OLs phenols to non-traditional olive tree product consumers or those refraining from sodium and fats. © 2023 Society of Chemical Industry.

Interactions of legume phenols-rice protein concentrate towards improving vegan food quality: Development of a protein-phenols enriched fruit smoothie.

Phenol-protein interaction is considered an effective tool to improve the functional properties of vegan proteins. The present work aimed to evaluate the covalent interaction between kidney bean polyphenols with rice protein concentrate and studied their characteristics for quality improvement in vegan-based foods. The impact of interaction on the techno-functional properties of protein was evaluated and the nutritional composition revealed that kidney bean was rich in carbohydrates. Furthermore, a noticeable antioxidant activity (58.11 ± 1.075 %) due to the presence of phenols (5.5 mg GAE/g) was observed for the kidney bean extract. Moreover, caffeic acid and p-Coumaric acid were confirmed using ultra-pressure liquid chromatography and the amount was 194.43 and 0.9272 mg/kg, respectively. A range of rice protein- phenols complexes (PPC0.025, PPC0.050, PPC0.075, PPC0.1, PPC0.2, PPC 0.5, PPC1) were examined and PPC0.2 and PPC0.5 showed significantly (p < 0.05) higher binding efficiency with proteins via covalent interaction. The conjugation reveals changes in physicochemical properties of rice protein, including, reduced size (178.4 nm) and imparted negative charges (-19.5 mV) of the native protein. The presence of amide Ⅰ, Ⅱ, Ⅲ, was confirmed in native protein and protein-phenol complex with vibration bands, particularly at 3784.92, 1631.07, and 1234 cm-1, respectively. The X-ray diffraction pattern depicted a slight decrease in crystallinity after the complexation and scanning electron microscopy revealed the alteration in morphology from less to improved smoothness and continuous surface characteristics for the complex. Thermo gravimetric analysis revealed high thermal stability of the complex with a maximum weight loss at a temperature range of 400-500 °C. Protein-phenol complex added fruit-based smoothie was developed and it was found to be acceptable in terms of various sensory attributes including color & appearance, textural consistency, and mouthfeel as compared to the control smoothie. Overall, this study provided novel insights to understand the phenol-protein interactions and the possible use of the phenol-rice protein complex in the development of vegan-based food products.

[Study of 2,6-di(propan-2-yl)phenol stability in biomaterial]. / Izuchenie ustoichivosti 2,6-di(propan-2-il)fenola v biologicheskom materiale.

The aim of this study is to research the stability of 2.6-di(propan-2-yl)phenol in biomaterial. GC-MS (column DB-5MS EVIDEX (25 m×0.2 mm); stationary liquid phase of 5%-phenyl-95% dimethylpolysiloxane), TLC (Sorbfil plates, mobile phase of hexane-diethyl ether (9:1) and spectrophotometry (solvent medium - 95% ethanol) were used as methods of analysis. 2.6-di(propan-2-yl)phenol was isolated from the biomatrix (liver tissue) by infusion with a mixture of ethyl acetate-acetone (7:3). The analyte was purified by combining extraction (water-ethyl acetate system) and semi-preparative chromatography on a column of silica gel L 40/100 µm, eluent - hexane-acetone (7:3). It was found that at -22 °C, 0 °C, 12 °C, 20 °C and 30 °C 2.6-di(propan-2-yl)phenol can be present in the liver tissue for 119, 98, 70, 56 and 42 days, respectively. The possibility of mathematical description of analyte decomposition dynamics in biomaterial (liver tissue) at the considered temperatures on the basis of hyperbola equation has been studied. The experimentally calculated coefficients in the hyperbola equation (km) for temperatures -22 °C, 0 °C, 12 °C, 20 °C and 30 °C are equal to 1823, 1130, 697, 510, and 255, respectively. The dependence km on the conserving temperature (tо) was educed. The equation for the description of dependence is offered: km=30.61∙(50-to)-402.39. It is shown that this equation can be the basis for prediction of 2.6-di(propan-2-yl)phenol stability in biomaterial (liver tissue) in the temperature range from -22 °C to 30 °C.

The reaction pathway and mechanism of 2,4-dichlorophenol removal by modified fly ash-loaded nZVI/Ni particles.

Nanoscale zero-valent iron (nZVI) is more valuable in environmental restoration than other materials. Chemical treatment of fly ash (CFA) was employed as a support material to disperse iron nickel bimetal nanoparticles (CFA-nZVI/Ni) to remove 2,4-dichlorophenol (2,4-DCP). Batch experiments showed that 2,4-DCP was completely removed by CFA-nZVI/Ni, and an optimal loading ratio was 8:1. The degradation of 2,4-DCP by CFA-nZVI/Ni was a chemical control reaction with an activation energy of 95.6 kJ mol-1 and followed pseudo-first-order kinetics. The addition of Cl- increased the removal rate of 2,4-DCP by 4%, while the addition of CO32- and SO42- decreased the removal rate of 2,4-DCP by 32% and 72.3%, respectively. The removal process of 2,4-DCP by CFA-nZVI/Ni included adsorption and reduction. The 2-CP (7.1 mg/L) and 4-CP (11.6 mg/L) could be converted to phenol using the CFA-nZVI/Ni system. Cl on the para-position of 2,4-DCP was simpler to remove than on the ortho-position. The following steps were taken in the electrophilic substitution reaction between substituted phenols and hydrogen radicals: 2,4-DCP > 2-CP > 4-CP > phenol. This research provides a novel concept to effectively remove 2,4-DCP and mechanism analysis.

Pollution and risk assessment of phenolic compounds in drinking water sources from South-Western Nigeria.

This study reports the occurrence and risk assessment of 2,4-dinitrophenol (2,4-DNP), phenol (PHE), and 2,4,6-trichlorophenol (2,4,6-TCP) in drinking water sources in three south-western States in Nigeria (Osun, Oyo, and Lagos). Groundwater (GW) and surface water (SW) were collected during dry and rainy seasons of a year. The detection frequency of the phenolic compounds followed the trend Phenol > 2,4-DNP > 2,4,6-TCP. The mean concentrations of 2,4-DNP, Phenol, and 2,4,6-TCP in GW/SW samples from Osun State were 639/553 µg L-1, 261/262 µg L-1, and 169/131 µg L-1 during the rainy season and 154/7 µg L-1, 78/37 µg L-1, and 123/15 µg L-1 during the dry season, respectively. In Oyo State, the mean concentrations were 165/391 µg L-1 for 2,4-DNP and 71/231 µg L-1 for Phenol in GW/SW samples, respectively, during the rainy season. Generally, in the dry season, these values decreased. In any case, these concentrations are higher than those previously reported in water from other countries. The concentration of 2,4-DNP in water posed serious ecological risks to Daphnia on the acute scale while it was algae on the chronic scale. Estimated daily intake and hazard quotient calculations suggest that 2,4-DNP and 2,4,6-TCP in water pose serious toxicity concerns to humans. Additionally, the concentration of 2,4,6-TCP in water from Osun State in both seasons of the year and in both groundwater and surface water poses significant carcinogenic risks to persons ingesting water from these sources in the State. Every exposure group studied were at risk from ingesting these phenolic compounds in water. However, this risk decreased with increasing age of the exposure group. Results from the principal component analysis indicate that 2,4-DNP in water samples is from an anthropogenic source different from that for Phenol and 2,4,6-TCP. There is a strong need to treat water from GW and SW systems in these States before ingesting while assessing their quality regularly.

Bamboo-derived nitrogen-doping magnetic porous hydrochar coactivated by K2FeO4 and CaCO3 for phenol removal: Governing factors and mechanisms.

In this study, a novel nitrogen-doped magnetic Fe-Ca codoped biochar for phenol removal was successfully fabricated via a hydrothermal and coactivation pyrolysis method. A series of adsorption process parameters (K2FeO4 to CaCO3 ratio, initial phenol concentration, pH value, adsorption time, adsorbent dosage and ion strength) and adsorption models (kinetic models, isotherms and thermodynamic models) were determined using batch experiments and various analysis techniques (XRD, BET, SEM-EDX, Raman spectroscopy, VSM, FTIR and XPS) to investigate the adsorption mechanism and metal-nitrogen-carbon interaction. The biochar with a ratio of Biochar: K2FeO4: CaCO3 = 3:1:1 exhibited superior properties for adsorption of phenol and had a maximum adsorption capacity of 211.73 mg/g at 298 K, C0 = 200 mg/L, pH = 6.0 and t = 480 min. These excellent adsorption properties were due to superior physicomechanical properties (a large specific surface area (610.53 m2/g) and pore volume (0.3950 cm3/g), a well-developed pore structure (hierarchical), a high graphitization degree (ID/IG = 2.02), the presence of O/N-rich functional groups and Fe-Ox,Ca-Ox, N-doping, as well as synergistic activation by K2FeO4 and CaCO3). The Freundlich and pseudo-second-order models effectively fit the adsorption data, indicating multilayer physicochemical adsorption. Pore filling and π-π interactions were the predominant mechanisms for phenol removal, and H-bonding interactions, Lewis-acid-base interactions, and metal complexation played an important role in enhancing phenol removal. A simple, feasible approach with application potential to organic contaminant/pollutant removal was developed in this study.

Associations between exposure to phthalates, phenols, and parabens with objective and subjective measures of sleep health among Mexican women in midlife: a cross-sectional and retrospective analysis.

Endocrine-disrupting chemicals (EDCs) may impact sleep during the menopausal transition by altering sex hormones. However, these studies are scarce among Latin American women. This investigation utilized cross-sectional and retrospective data from midlife women enrolled in the Early Life Exposure in Mexico to Environmental Toxicants (ELEMENT) study to examine associations between exposure to EDCs (phthalates, phenols, and parabens) and sleep health measures. For cross-sectional analyses, single spot urine samples were collected between 2017-2019 from a pilot sample of women (N = 91) of midlife age to estimate the urinary concentration of individual phthalates, phenols, and parabens and to calculate the summary concentration of phthalate mixtures. Seven-day nightly sleep duration, midpoint, and fragmentation were obtained from wrist-actigraphy devices and estimated from the actigraphy data using a pruned dynamic programming algorithm. Self-reported poor sleep quality was assessed by one item from the Pittsburgh Sleep Quality Index (PSQI). We examined associations between urinary summary phthalate mixtures, phthalate metabolites, phenol, and paraben analytes with each sleep measure using linear or logistic (to compute odds of poor sleep quality only) regression models adjusted for specific gravity, age, and socioeconomic status. We ran similar regression models for retrospective analyses (N = 74), except that urine exposure biomarker data were collected in 2008 when women were 24-50 years old. At the 2017-2019 midlife visit, 38% reported poor sleep quality. Cross-sectionally, EDCs were associated with longer sleep duration, earlier sleep timing, and more fragmented sleep. For example, every 1-unit IQR increase in the phenol triclosan was associated with a 26.3 min per night (95% CI: 10.5, 42.2; P < 0.05) longer sleep duration and marginally associated with 0.2 decimal hours (95% CI: -0.4, 0.0; P < 0.10) earlier sleep midpoint; while every 1-unit IQR increase in the phthalate metabolite MEHP was associated with 1.1% higher sleep fragmentation (95% CI: 0.1, 2.1; P < 0.05). Retrospective study results generally mirrored cross-sectional results such that EDCs were linked to longer sleep duration, earlier sleep timing, and more fragmented sleep. EDCs were not significantly associated with odds of self-reported poor sleep quality. Results from cross-sectional and retrospective analyses revealed that higher exposure to EDCs was predictive of longer sleep duration, earlier sleep timing, and more fragmented sleep among midlife women.

Systematic studies on the effect of structural modification of orange peel for remediation of phenol contaminated water.

In the present study, orange peel biochar has been utilized as the adsorbent for the removal of phenol from contaminated water. The biochar was prepared by thermal activation process at three different temperature 300, 500 and 700°C and are defined as B300, B500, and B700 respectively. The synthesized biochar has been characterized using scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transformation infrared spectroscopy (FTIR), RAMAN spectroscopy, X-ray photoelectron spectroscopy (XPS), and UV-Vis spectroscopy. SEM analysis revealed a highly irregular and porous structure for B700 as compared with others. The parameters such as initial phenol concentration, pH, adsorption dosage, and contact time were optimized, and the maximum adsorption efficiency and capacity of about 99.2% and 31.0 mg/g was achieved for B700 for phenol adsorption. The Branauer-Emmett-Teller (BET) surface area and Berrate-Joyner-Halenda (BJH) pore diameter obtained for B700 were about 67.5 m2 /g and 3.8 nm. The adsorption of phenol onto the biochar followed Langmuir isotherm showing linear fit with R2 = 0.99, indicating monolayer adsorption. The kinetic data for adsorption is best fitted for pseudo-second order. The thermodynamic parameters ΔG°, ΔH°, and ΔS° values obtained are negative, which means that the adsorption process is spontaneous and exothermic. The adsorption efficiency of phenol marginally declined from 99.2% to 50.12% after five consecutive reuse cycles. The study shows that the high-temperature activation increased the porosity and number of active sites over the orange peel biochar for efficient adsorption of phenol. PRACTITIONER POINTS: Orange peel is thermally activated at 300, 500, and 700°C for structure modification. Orange peel biochars were characterized for its structure, morphology, functional groups, and adsorption behavior. High-temperature activation improved the adsorption efficiency up to 99.21% due to high porosity.

Wild blueberry (poly)phenols can improve vascular function and cognitive performance in healthy older individuals: a double-blind randomized controlled trial.

BACKGROUND: Evidence suggests that the intake of blueberry (poly)phenols is associated with improvements in vascular function and cognitive performance. Whether these cognitive effects are linked to increases in cerebral and vascular blood flow or changes in the gut microbiota is currently unknown. METHODS: A double-blind, parallel randomized controlled trial was conducted in 61 healthy older individuals aged 65-80 y. Participants received either 26 g of freeze-dried wild blueberry (WBB) powder (302 mg anthocyanins) or a matched placebo (0 mg anthocyanins). Endothelial function measured by flow-mediated dilation (FMD), cognitive function, arterial stiffness, blood pressure (BP), cerebral blood flow (CBF), gut microbiome, and blood parameters were measured at baseline and 12 wk following daily consumption. Plasma and urinary (poly)phenol metabolites were analyzed using microelution solid-phase extraction coupled with liquid chromatography-mass spectrometry. RESULTS: A significant increase in FMD and reduction in 24 h ambulatory systolic BP were found in the WBB group compared with the placebo group (0.86%; 95% CI: 0.56, 1.17, P < 0.001; -3.59 mmHg; 95% CI: -6.95, -0.23, P = 0.037; respectively). Enhanced immediate recall on the auditory verbal learning task, alongside better accuracy on a task-switch task was also found following WBB treatment compared with placebo (P < 0.05). Total 24 h urinary (poly)phenol excretion increased significantly in the WBB group compared with placebo. No changes in the CBF or gut microbiota composition were found. CONCLUSIONS: Daily intake of WBB powder, equivalent to 178 g fresh weight, improves vascular and cognitive function and decreases 24 h ambulatory systolic BP in healthy older individuals. This suggests that WBB (poly)phenols may reduce future CVD risk in an older population and may improve episodic memory processes and executive functioning in older adults at risk for cognitive decline. Clinical Trial Registration number in clinicaltrials.gov: NCT04084457.

Biotinylated caffeic acid covalent binding with myofibrillar proteins in alkaline conditions: Identification of protein-phenol adducts and alterations in protein properties.

In this study, the effects of covalent interactions between myofibrillar proteins (MP) and caffeic acid (CA) were investigated. Protein-phenol adducts were identified by biotinylated caffeic acid (BioC) used as a substitution of CA. The total sulfhydryls and free amines content were decreased (p < 0.05). The α-helix structure of MP increased (p < 0.05) and MP gel properties enhanced slightly at low dosages of CA (10 and 50 µM), and both were impaired significantly (p < 0.05) at high dosages of CA (250 and 1250 µM). Two prominent adducts of myosin heavy chain (MHC)-BioC and Actin-BioC were identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), which gradually increased at low concentrations of BioC (10 and 50 µM), and raised significantly at the concentration of 1250 µM. According to the correlation analysis, MHC-BioC and Actin-BioC adducts showed a significant negative correlation with gel properties, such as G', hardness, and water holding capacity (WHC) (p < 0.01), which indicated that the covalent interactions between MP and CA significantly affected the quality of meat products.